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Genetic regulation involved in the geomicrobiology of manganese in the marine environment. ArchiMer
Colwell, R; Schuett, C; Zelibor, J.
Recent reports in the literature suggest a plasmid-encoded mechanism for manganese oxidation by bacteria. To elucidate the role, if any, of plasmids in bacterial manganese oxidation in the marine environment, manganese-oxidizing bacterial strains, isolated from manganese nodules, sediment and seawater samples collected from the Pacific Ocean and Mediterranean Sea were examined for the presence of plasmids and their association with manganese oxidation. 22 of 47 strains of the genera Alcaligenes, Pseudomonas , and Vibrio harbored single plasmids of various sizes even after prolonged storage. The enzymatic oxidation of manganese by some marine bacteria may not be exclusively encoded by plasmid DNA. Functions ascribable to the plasmids remain cryptic,...
Tipo: Text Palavras-chave: MED I; Pacific Sea water Sediments Bacteria Biogeochemistry Ferromanganese nodules Genomes Oxidation Manganese.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-981.pdf
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Plasmid-associated phenathrene degradation by Chesapeake Bay sediment bacteria. ArchiMer
Okpokwasili, G; Somerville, C; Grimes, D; Colwell, R.
A differential, phenanthrene-enrichment agar plating technique was used to isolate phenanthrene-degrading bacteria from phenanthrene-enriched Chesapeake Bay sediment. One of the isolates, a yellow pigmented, slime-producing, Gram-negative rod identified as Flavobacterium sp. has been studied in detail. It has been found to carry a single plasmid with a mass of about 34 megadaltons. Results of hydrocarbon adherence tests showed that the organism adhered only minimally to n-octane and n-hexadecane, but emulsified cyclohexylbenzene and 1,2,3,4,-tetrahydronaphthalene (tetralin). Curing of the plasmid with 3 mu g/ml novobiocin resulted in loss of phenanthrene clearing ability. Ability to degrade phenanthrene was confirmed using super(14)C-labeled phenanthrene.
Tipo: Text Palavras-chave: Flavobacterium; Microbiological culture; Culture media; Bacteria; Sediment pollution; Biodegradation; Bacteria collecting devices.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1014.pdf
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